An immunostimulatory polysaccharide (SCP-IIa) from the fruit of Schisandra chinensis (Turcz.) Baill

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• 作者：Yong Chen^a ; Jinbao Tang^b ; ¹ ; ^{tangjb@wfmc.edu.cn} ; Xiaoke Wang^a ; Fengxiang Sun^a ; Shujuan Liang^a ; ¹ ; ^{liangshjuan@yahoo.cn}
• 关键词：Polysaccharide ; Schisandra chinensis ; Immunomodulatory effect ; Cyclophosphamide
• 刊名：International Journal of Biological Macromolecules
• 出版年：2012
• 出版时间：1 April, 2012
• 年：2012
• 卷：50
• 期：3
• 页码：844-848
• 全文大小：396 K

文摘

A water-soluble polysaccharide named SCP-IIa was isolated from the water extract of the fruit of Schisandra chinensis (Turcz.) Baill by means of ethanol precipitation, deproteination, anion-exchange and gel-permeation chromatography. The molecular weight of SCP-IIa was ascertained via HPLC, and immuno-modulating effect was evaluated using the immunosuppressed model induced by cyclophosphamide. SCP-IIa was a homogeneous form of polysaccharide, with an average molecular weight of approximately 7700 Da. The detected parameters showed that SCP-IIa increased the thymus and spleen indices, as well as the pinocytic activity of the peritoneal macrophages in immunosuppressed mice. The splenocyte proliferation assay showed that SCP-IIa, in combination with Con A or LPS, positively affected splenocyte proliferation. Moreover, the polysaccharide promoted hemolysin formation. The results suggested that SCP-IIa was involved in immunomodulatory effects leading to the exploration for SCP-IIa as a potential immunostimulant.