Caged DNA Does Not Aggregate in High Ionic Strength Solutions

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• 作者：Vladimir S. Trubetskoy ; Aaron Loomis ; Paul M. Slattum ; James E. Hagstrom ; Vladimir G. Budker ; and Jon A. Wolff
• 刊名：Bioconjugate Chemistry
• 出版年：1999
• 出版时间：July 1999
• 年：1999
• 卷：10
• 期：4
• 页码：624 - 628
• 全文大小：106K
• 年卷期：v.10,no.4(July 1999)
• ISSN：1520-4812

文摘

The assembly of DNA into compact particles that do not aggregate in physiologic salt solution occursnaturally in chromatin and viral particles but has been challenging to duplicate using artificialconstructs. Cross-linking amino-containing polycations in the presence of DNA with bisimidoestercross-linker leads to the formation of caged DNA particles that are stable in salt solutions. This firstdemonstration of caged DNA provides insight into how natural condensation processes avoidaggregation and a promising avenue for developing nonviral gene therapy vectors.