Selective Cell Attachment to a Biomimetic Polymer Surface through the Recognition of Cell-Surface Tags

详细信息    查看全文

• 作者：Yasuhiko Iwasaki ; Emi Tabata ; Kimio Kurita ; and Kazunari Akiyoshi
• 刊名：Bioconjugate Chemistry
• 出版年：2005
• 出版时间：May 2005
• 年：2005
• 卷：16
• 期：3
• 页码：567 - 575
• 全文大小：406K
• 年卷期：v.16,no.3(May 2005)
• ISSN：1520-4812

文摘

Reactive phosphorylcholine polymers, which can recognize biosynthetic cell-surface tags, weresynthesized to control cell attachment. Human promyelocytic leukemia cells (HL-60) with unnaturalcarbohydrates as cell-surface tags were harvested by treatment with N-levulinoylmannosamine(ManLev). The attachment of ManLev-treated HL-60 cells to 2-methacryloyloxyethyl phosphorylcholine(MPC) polymers with hydrazide groups was studied. HL-60 cells, which are nonadhesive, did notattach to any polymer surface without ManLev treatment. In contrast, ManLev-treated HL-60 cellsattached to a poly[MPC-co-n-butyl methacrylate (BMA)-co-methacryloyl hydrazide (MH)] (PMBH)surface following 15 min of incubation. The cells that attached to the PMBH surface retained theirnative morphology and viability for 24 h of incubation. On the other hand, approximately half of theHL-60 cells that attached to the poly(BMA-co-MH) (PBH) surface died. These results suggest thatMH units in the polymer act as anchors for cell attachment and MPC units help to preserve cellviability on a polymer surface. The coculture of ManLev-treated HL-60 and fluorescence-stained humanuterine cervical cancer cells (HeLa) was carried out on polymer surfaces. ManLev-treated HL-60 cellsspecifically attached to the PMBH surface. In contrast, both HL-60 and HeLa cells were observed onthe PBH surface. The control of cellular interactions with synthetic polymers may be useful for thefuture development of cell-integrated biosensors and biomedical devices.