Encoded Silica Colloidal Crystal Beads as Supports for Potential Multiplex Immunoassay

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• 作者：Yuanjin Zhao ; Xiangwei Zhao ; Cheng Sun ; Juan Li ; Rong Zhu ; Zhongze Gu
• 刊名：Analytical Chemistry
• 出版年：2008
• 出版时间：March 1, 2008
• 年：2008
• 卷：80
• 期：5
• 页码：1598 - 1605
• 全文大小：676K
• 年卷期：v.80,no.5(March 1, 2008)
• ISSN：1520-6882

文摘

We developed a new kind of suspension array for multiplexed immunoassays using silica colloidal crystal beads(SCCBs) as coding carriers. The monodisperse and size-controlled SCCBs were fabricated by a microfluidic device.Calcination was employed to improve the mechanicalstability and lower the fluorescent background of theSCCBs. Immobilization of protein molecules on the surface of the SCCBs through chemical bonds was studied,and the modification condition was optimized to increasethe detection sensitivity. Results indicated that the SCCBsas supports were more sensitive (0.92 ng/mL IgG) thanthe glass beads (27 ng/mL IgG) and the planar carriers(140 ng/mL IgG). A multiplex immunoassay showed theflexibility and feasibility of SCCBs array in clinical applications.