Novel Strategy to Release and Tag N-Glycans for Functional Glycomics

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• 作者：Xuezheng Song ; Hong Ju ; Chunmei Zhao ; Yi Lasanajak
• 刊名：Bioconjugate Chemistry
• 出版年：2014
• 出版时间：October 15, 2014
• 年：2014
• 卷：25
• 期：10
• 页码：1881-1887
• 全文大小：467K
• ISSN：1520-4812

文摘

Functional glycomics has been impeded by the lack of inexpensive enzymatic and mild chemical methods to acquire natural glycans in significant amounts. In this study, we have developed a new strategy we term 鈥渢hreshing and trimming鈥?(TaT) to quickly obtain N-glycans from glycoproteins and animal tissues. TaT employs low-cost Pronase to degrade peptides and N-bromosuccinimide (NBS) to effect oxidative decarboxylation under very mild reaction conditions to generate homogeneous aglycon moieties as nitriles or aldehydes. These aglycons can be readily conjugated with fluorescent tags for profiling and functional study. TaT is an affordable alternative to expensive specialty enzymes and strong chemical treatment and unpleasant reagents, and should further drive the functional glycomics of N-glycans.