Self-Sensing Porphysomes for Fluorescence-Guided Photothermal Therapy

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• 作者：Kenneth K. Ng ; Misa Takada ; Cheng C.S. Jin ; Gang Zheng
• 刊名：Bioconjugate Chemistry
• 出版年：2015
• 出版时间：February 18, 2015
• 年：2015
• 卷：26
• 期：2
• 页码：345-351
• 全文大小：358K
• ISSN：1520-4812

文摘

Porphysomes are highly quenched unilamellar porphyrin鈥搇ipid nanovesicles with structurally dependent photothermal properties. The high packing density of porphyrin molecules in the lipid bilayer enables their application in photothermal therapy, whereas the partial disruption of the porphysome structure over time restores the porphyrin fluorescence and enables the fluorescence-guided photothermal ablation. This conversion is a time-dependent process and cannot be easily followed using existing analytical techniques. Here we present the design of a novel self-sensing porphysome (FRETysomes) capable of fluorescently broadcasting its structural state through F枚rster resonance energy transfer. By doping in a near-infrared emitting fluorophore, it is possible to divert a small fraction of the absorbed energy toward fluorescence emission which provides information on whether the vesicle is intact or disrupted. Addition of bacteriopheophorbide鈥搇ipid into the vesicle bilayer as a fluorescence acceptor (0.5鈥?.5 mol %) yields a large separation of 100 nm between the absorption and fluorescence bands of the nanoparticle. Furthermore, a progressive increase in FRET efficiency (14.6鈥?2.7%) is observed. Photothermal heating and serum stability in FRETysomes is comparable with the undoped porphysomes. The fluorescence arising from the energy transfer between the donor and acceptor dyes can be clearly visualized in vivo through hyperspectral imaging. By calculating the ratio between the acceptor and donor fluorescence, it is possible to determine the structural fate of the nanovesicles. We observe using this technique that tumor accumulation of structurally intact porphyrin鈥搇ipid nanovesicles persists at 24 and 48 h postinjection. The development of FRETysomes offers a unique and critical imaging tool for planning porphysome-enabled fluorescence-guided photothermal treatment, which maximizes light-induced thermal toxicity.