Noninvasive Acoustic Cell Trapping in a Microfluidic Perfusion System for Online Bioassays
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- 作者：Mikael Evander ; Linda Johansson ; Tobias Lilliehorn ; Jure Piskur ; Magnus Lindvall ; Stefan Johansson ; Monica Almqvist ; Thomas Laurell ; Johan Nilsson
- 刊名：Analytical Chemistry
- 出版年：2007
- 出版时间：April 1, 2007
- 年：2007
- 卷：79
- 期：7
- 页码：2984 - 2991
- 全文大小：578K
- 年卷期：v.79,no.7(April 1, 2007)
- ISSN：1520-6882
文摘
Techniques for manipulating, separating, and trappingparticles and cells are highly desired in today's bioanalytical and biomedical field. The microfluidic chip-basedacoustic noncontact trapping method earlier developedwithin the group now provides a flexible platform forperforming cell- and particle-based assays in continuousflow microsystems. An acoustic standing wave is generated in etched glass channels (600 × 61 
m2) byminiature ultrasonic transducers (550 × 550 × 200m3). Particles or cells passing the transducer will beretained and levitated in the center of the channel withoutany contact with the channel walls. The maximum trapping force was calculated to be 430 ± 135 pN bymeasuring the drag force exerted on a single particlelevitated in the standing wave. The temperature increasein the channel was characterized by fluorescence measurements using rhodamine B, and levels of moderatetemperature increase were noted. Neural stem cells wereacoustically trapped and shown to be viable after 15 min.Further evidence of the mild cell handling conditions wasdemonstrated as yeast cells were successfully cultured for6 h in the acoustic trap while being perfused by the cellmedium at a flowrate of 1 L/min. The acoustic microchipmethod facilitates trapping of single cells as well as largercell clusters. The noncontact mode of cell handling isespecially important when studies on nonadherent cellsare performed, e.g., stem cells, yeast cells, or blood cells,as mechanical stress and surface interaction are minimized. The demonstrated acoustic trapping of cells andparticles enables cell- or particle-based bioassays to beperformed in a continuous flow format.
m2) byminiature ultrasonic transducers (550 × 550 × 200m3). Particles or cells passing the transducer will beretained and levitated in the center of the channel withoutany contact with the channel walls. The maximum trapping force was calculated to be 430 ± 135 pN bymeasuring the drag force exerted on a single particlelevitated in the standing wave. The temperature increasein the channel was characterized by fluorescence measurements using rhodamine B, and levels of moderatetemperature increase were noted. Neural stem cells wereacoustically trapped and shown to be viable after 15 min.Further evidence of the mild cell handling conditions wasdemonstrated as yeast cells were successfully cultured for6 h in the acoustic trap while being perfused by the cellmedium at a flowrate of 1 L/min. The acoustic microchipmethod facilitates trapping of single cells as well as largercell clusters. The noncontact mode of cell handling isespecially important when studies on nonadherent cellsare performed, e.g., stem cells, yeast cells, or blood cells,as mechanical stress and surface interaction are minimized. The demonstrated acoustic trapping of cells andparticles enables cell- or particle-based bioassays to beperformed in a continuous flow format.© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |