Solution NMR of Large Molecules and Assemblies

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• 作者：Mark P. Foster ; Craig A. McElroy ; Carlos D. Amero
• 刊名：Biochemistry
• 出版年：2007
• 出版时间：January 16, 2007
• 年：2007
• 卷：46
• 期：2
• 页码：331 - 340
• 全文大小：607K
• 年卷期：v.46,no.2(January 16, 2007)
• ISSN：1520-4995

文摘

Solution NMR spectroscopy represents a powerful tool for examining the structure and functionof biological macromolecules. The advent of multidimensional (2D-4D) NMR, together with the widespreaduse of uniform isotopic labeling of proteins and RNA with the NMR-active isotopes, ¹⁵N and ¹³C, openedthe door to detailed analyses of macromolecular structure, dynamics, and interactions of smallermacromolecules (<~25 kDa). Over the past 10 years, advances in NMR and isotope labeling methodshave expanded the range of NMR-tractable targets by at least an order of magnitude. Here we brieflydescribe the methodological advances that allow NMR spectroscopy of large macromolecules and theircomplexes and provide a perspective on the wide range of applications of NMR to biochemical problems.