A Stereocomplex Platform Efficiently Detecting Antigen-Antibody Interactions

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• 作者：Takeshi Serizawa ; Yuya Nagasaka ; Hisao Matsuno ; Masakazu Shimoyama ; Kimio Kurita
• 刊名：Bioconjugate Chemistry
• 出版年：2007
• 出版时间：March 2007
• 年：2007
• 卷：18
• 期：2
• 页码：355 - 362
• 全文大小：302K
• 年卷期：v.18,no.2(March 2007)
• ISSN：1520-4812

文摘

Ultrathin poly(methyl methacrylate) (PMMA) stereocomplex films with macromolecularly double-stranded regularnanostructures were prepared by layer-by-layer assembly of isotactic and syndiotactic PMMAs on solid surfaces.Antibodies were immobilized through the Fc region-capturing protein A, which had been physically adsorbed onthe complex film, and the binding of antigens to immobilized antibodies was quantitatively investigated by thequartz crystal microbalance technique. Greater amounts of protein A with native forms were adsorbed on thecomplex film than those on conventional single-component PMMA films. Antibodies with high target-bindingactivities were also immobilized on the complex film. A greater amount of antigens could be detected on thecomplex film. The activity of protein A was maintained on the complex for a long time even within a dried state.The mechanism for the preservation of protein native forms on the complex surface was speculated by analyzingthe physical adsorption of proteins with various secondary structures. Stereocomplex films can be utilized asnovel coating nanomaterials for efficiently detecting protein-protein interactions.