Asymmetric Reduction of o-Chloroacetophenone with Candida pseudotropicalis 104

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• 作者：Qing Xie ; Jianping Wu ; Gang Xu ; Lirong Yang
• 刊名：Biotechnology Progress
• 出版年：2006
• 出版时间：October 2006
• 年：2006
• 卷：22
• 期：5
• 页码：1301 - 1304
• 全文大小：111K
• 年卷期：v.22,no.5(October 2006)
• ISSN：1520-6033

文摘

The asymmetric reduction of o-chloroacetophenone 1 with Candida pseudotropicalis 104produced the corresponding (S)-1-(2-chloro-phenyl)-ethanol 2 with the enantiomeric excess (ee>99%) without addition of any cosolvent. The cell could tolerate high ketone 1 concentrationof 233.8 mmol/L (i.e., 36 g/L) with considerable reduction activity in this method. The product2 concentration achieved 38.9 and 58.4 mmol/L with cells of 40 and 60 g_DCW (dry cell weight)/L, respectively, in 24 h. The optimum reaction time, the effect of substrate concentration,cosubstrate type and concentration, and cell concentration in the reaction were investigated inthis paper.