Chitosan Graft Copolymer Nanoparticles for Oral Protein Drug Delivery: Preparation and Characterization

详细信息    查看全文

• 作者：Feng Qian ; Fuying Cui ; Jieying Ding ; Cui Tang ; Chunhua Yin
• 刊名：Biomacromolecules
• 出版年：2006
• 出版时间：October 2006
• 年：2006
• 卷：7
• 期：10
• 页码：2722 - 2727
• 全文大小：151K
• 年卷期：v.7,no.10(October 2006)
• ISSN：1526-4602

文摘

Several novel functionalized graft copolymer nanoparticles consisting of chitosan (CS) and the monomer methylmethacrylate (MMA), N-dimethylaminoethyl methacrylate hydrochloride (DMAEMC), and N-trimethylaminoethylmethacrylate chloride (TMAEMC), which show a higher solubility than chitosan in a broader pH range, havebeen prepared by free radical polymerization. The nanoparticles were characterized in terms of particle size, zetapotential, TEM, and FT-IR. These nanoparticles were 150-280 nm in size and carried obvious positive surfacecharges. Protein-loaded nanoparticles were prepared, and their maximal encapsulation efficiency was up to 100%.In vitro release showed that these nanoparticles provided an initial burst release followed by a slowly sustainedrelease for more than 24 h. These graft copolymer nanoparticles enhanced the absorption and improved thebioavailability of insulin via the gastrointestinal (GI) tract of normal male Sprague-Dawley (SD) strain rats toa greater extent than that of the phosphate buffer solution (PBS) of insulin.