Propagation of Blood Clotting in the Complex Biochemical Network of Hemostasis Is Described by a Simple Mechanism

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• 作者：Matthew K. Runyon ; Bethany L. Johnson-Kerner ; Christian J. Kastrup ; Thuong G. Van Ha ; Rustem F. Ismagilov
• 刊名：Journal of the American Chemical Society
• 出版年：2007
• 出版时间：June 6, 2007
• 年：2007
• 卷：129
• 期：22
• 页码：7014 - 7015
• 全文大小：94K
• 年卷期：v.129,no.22(June 6, 2007)
• ISSN：1520-5126

文摘

Hemostasis is the complex biochemical network that controls blood clotting. We previously described a chemical model that mimicked the dynamics of hemostasis based on a simple regulatory mechanism-a threshold response due to the competition between production and removal of activators. Here, we used human blood plasma in phospholipid-coated microfluidic channels to test predictions based on this mechanism. We demonstrated that, for a given geometry of channels, clot propagation from an obstructed channel into a channel with flowing blood plasma is dependent on the shear rate in the channel with flowing blood plasma. If confirmed in vivo, these results may explain clot propagation from a small vessel to a larger, clinically relevant vessel. In addition, these results would further validate the use of modular mechanisms, simplified chemical models, and microfluidics to study complex biochemical networks.