Assembly of Three-Dimensional Polymeric Constructs Containing Cells/Biomolecules Using Carbon Dioxide

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• 作者：Yong Yang ; Yubing Xie ; Xihai Kang ; L. James Lee ; Douglas A. Kniss
• 刊名：Journal of the American Chemical Society
• 出版年：2006
• 出版时间：November 1, 2006
• 年：2006
• 卷：128
• 期：43
• 页码：14040 - 14041
• 全文大小：141K
• 年卷期：v.128,no.43(November 1, 2006)
• ISSN：1520-5126

文摘

Using low-pressure carbon dioxide (CO₂), we demonstrated a novel and versatile approach to assembling polymeric constructs in the presence of cells and/or biomolecules in an aqueous environment. By regulating the CO₂ pressure, the assembly was completed at biologically permissive temperatures with excellent preservation of the original structures. We further demonstrated that mammalian cells can survive the CO₂-assisted bioassembly process (37 C, 1.38 MPa, ~1 h). Human mesenchymal stem cells from bone marrow (hMSCs) exhibited the same cell morphology and proliferation potential as the untreated control. Mouse embryonic stem cells (mESCs) maintained ES-specific Oct-4 gene expression and differentiation potential after CO₂ treatment as well. This method highlights the ability to construct multiple biodegradable polymeric scaffolds with well-defined architecture, on which various types of cells were grown, into a predesigned three-dimensional complex. In addition, protein and DNA bioactivity can be preserved in the context of a CO₂-assisted assembly. This CO₂-assisted bioassembly method provides for a manufacturing platform that, thus far, has been lacking in the fields of tissue engineering, cell-based biochips, cell therapy, and drug delivery.