20S-Protopanaxadiol-Induced Programmed Cell Death in Glioma Cells through Caspase-Dependent and -Independent Pathways

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• 作者：Guo-Yu Liu ; Xuexian Bu ; Hang Yan ; William W.-G. Jia
• 刊名：Journal of Natural Products
• 出版年：2007
• 出版时间：February 2007
• 年：2007
• 卷：70
• 期：2
• 页码：259 - 264
• 全文大小：537K
• 年卷期：v.70,no.2(February 2007)
• ISSN：1520-6025

文摘

20S-Protopanaxadiol (1) is an aglycon metabolic derivative of the protopanaxadiol-type ginseng saponins. In the presentstudy, 1 was used to induce cytotoxicity for two human glioma cell lines, SF188 and U87MG. For the SF188 cells, 1activated caspases-3, -8, -7, and -9 within 3 h and induced rapid apoptosis, which could be partially inhibited by ageneral caspase blocker and completely abolished when the caspase blocker was used in combination with an antioxidant.Compound 1 also induced cell death in U87MG cells but did not activate any caspases in these cells. Monodansylcadaverine staining showed that 1 induced dramatic autophagy in both cell lines. Elevated levels of superoxide anion inboth cells and reduced levels of phosphorylated Akt in U87MG cells were also demonstrated. These results showed that20S-protopanaxadiol (1) induces different forms of programmed cell death, including both typical apoptosis and autophagythrough both caspase-dependent and -independent mechanisms.