Proteome Analysis and Tissue Microarray for Profiling Protein Markers Associated with Lymph Node Metastasis in Colorectal Cancer

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• 作者：Haiping Pei ; Hong Zhu ; Shan Zeng ; Yixiong Li ; Huixiang Yang ; Liangfang Shen ; Jia Chen ; Liang Zeng ; Jianghong Fan ; ^O ; Xiaogang Li ; Yuewen Gong ; ^O ; Hong Shen
• 刊名：Journal of Proteome Research
• 出版年：2007
• 出版时间：July 2007
• 年：2007
• 卷：6
• 期：7
• 页码：2495 - 2501
• 全文大小：701K
• 年卷期：v.6,no.7(July 2007)
• ISSN：1535-3907

文摘

Background: Understanding the proteins associated with lymph node metastasis (LNM) in colorectalcancer (CRC) will benefit us in the prediction of CRC prognosis and provide us new potential targets inthe intervention of CRC. The aim of this study is to investigate the LNM-associated proteins and toevaluate the clinicopathological characteristics of these target proteins' expression in CRC. Methods:Fresh tumor and paired normal mucosa from five cases for each group of non-LNM CRC and LNMCRC were analyzed by two-dimensional electrophoresis coupled with MALDI-TOF-MS, followed byWestern blotting confirmation. In 40 paraffin-embedded CRC samples, each for non-LNM CRC and LNMCRC, four differentially expressed proteins identified by proteomics analysis were detected by tissuemicroarray with immunohistochemistry staining to access the clinicopathological characteristics of theseproteins in LNM of CRC. Results: Twenty-five proteins were found to be differentially expressed betweennormal mucosa and CRC tissue. Increased expression levels of heat shock protein-27 (HSP-27),glutathione S-transferase (GST), and Annexin II, but a decreased expression level of liver-fatty acidbinding protein (L-FABP), existed in LNM CRC as compared with non-LNM CRC (p < 0.01 or p < 0.05,respectively). Conclusion: The techniques of proteomic analysis combined with tissue microarrayprovide us a dramatic tool for screening of LNM-associated proteins in cancer research. The increasedexpression of HSP-27, GST, and Annexin II, but decreased expression of L-FABP, suggests a significantlyelevated incidence of LNM in CRC.