Proteomic and Peptidomic Characterization of the Venom from the Chinese Bird Spider, Ornithoctonus huwena Wang

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• 作者：Chunhua Yuan ; Qihui Jin ; Xing Tang ; Weijun Hu ; Rui Cao ; Shengqing Yang ; Jixian Xiong ; Chunliang Xie ; Jinyun Xie ; Songping Liang
• 刊名：Journal of Proteome Research
• 出版年：2007
• 出版时间：July 2007
• 年：2007
• 卷：6
• 期：7
• 页码：2792 - 2801
• 全文大小：324K
• 年卷期：v.6,no.7(July 2007)
• ISSN：1535-3907

文摘

The bird spider Ornithoctonus huwena Wang is a very venomous spider in China. Several compoundswith different types of biological activities have been identified previously from the venom of this spider.In this study, we have performed a proteomic and peptidomic analysis of the venom. The venom waspreseparated into two parts: the venom proteins with molecular weight (MW) higher than 10 000 andthe venom peptides with MW lower than 10 000. Using one-dimensional gel electrophoresis (1-DE),two-dimensional gel electrophoresis (2-DE), and mass spectrometry, 90 proteins were identified,including some important enzymes, binding proteins, and some proteins with significant biologicalfunctions. For venom peptides, a combination of cation-exchange and reversed-phase chromatographywas employed. More than 100 components were detected by mass spectrometry, and 47 peptideswere sequenced by Edman degradation. The peptides display structural and pharmacological diversityand share little sequence similarity with peptides from other animal venoms, which indicates the venomof O. huwena Wang is unique. The venom peptides can be classified into several superfamilies. Alsoit is revealed that gene duplication and focal hypermutation have taken place during the evolution ofthe spider toxins.