The Alterations of Mouse Plasma Proteins during Septic Development

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• 作者：Yan Ren ; Jiandong Wang ; Ji Xia ; Chaoguang Jiang ; Kang Zhao ; Rong Li ; Ningzhi Xu ; Yingxin Xu ; Siqi Liu
• 刊名：Journal of Proteome Research
• 出版年：2007
• 出版时间：July 2007
• 年：2007
• 卷：6
• 期：7
• 页码：2812 - 2821
• 全文大小：451K
• 年卷期：v.6,no.7(July 2007)
• ISSN：1535-3907

文摘

A fundamental issue for sepsis therapy is to control the development of inflammation at an early stage.With cecal ligation and puncture (CLP) surgery, the mouse model has clearly shown the septic signstriggered by chronic insult. To monitor the plasma proteomic responses to sepsis, the mouse bloodwas collected at intervals after sham and CLP surgery followed by the sample treatment to removehigh abundance serum albumin. The treated mouse plasma proteins were well resolved by two-dimensional electrophoresis (2-DE). The image analysis revealed that these 2-DE spots observed fromthe sham and the CLP samples 4 h after surgery were comparable, whereas more than 30 differentspots appeared on the 2-DE gels between the sham and CLP mouse plasma 24 h after surgery, indicatingthat some plasma proteins responded to the inflammatory development. These differential spots wereverified by MALDI-TOF/TOF MS, resulting in 13 unique sepsis-responsive proteins. More importantly,most of them exhibited multiple spots as difference on the 2-DE gels. Furthermore, these isospotswere incubated with PNGase F to eliminate N-linked oligosaccharides on proteins and then evaluatedby Western blot as well as mass spectrometry. The results of PNGase F digestion suggested that mostsepsis-associated proteins remained in N-glycosylation status but changed their N-glycans during septicdevelopment.