Limited Use of Centritech Lab II Centrifuge in Perfusion Culture of rCHO Cells for the Production of Recombinant Antibody

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• 作者：Byoung Jin Kim ; Duk Jae Oh ; Ho Nam Chang
• 刊名：Biotechnology Progress
• 出版年：2008
• 出版时间：February 2008
• 年：2008
• 卷：24
• 期：1
• 页码：166 - 174
• 全文大小：344K
• 年卷期：v.24,no.1(February 2008)
• ISSN：1520-6033

文摘

Perfusion cultures of recombinant Chinese hamster ovary cells, producing recombinant antibodyagainst the S surface antigen of Hepatitis B virus, were carried out in continuous and intermittentmode using a Centritech Lab II Centrifuge. In the continuous perfusion process, despite theabsence of shear stress from the pump head, long-term operation was not possible because ofcontinuously repeated exposure to oxygen limitation and low temperature, as well as shear stressfrom centrifugal force. In the intermittent perfusion processes, the frequency of cell-passagethrough the centrifuge was substantially reduced, compared with the continuous perfusion mode;however, the degree of reduction could not guarantee stable long-term operation. Although variousoperating parameters were applied in the intermittent perfusion cultures, high cell densities couldnot be maintained stably. In a single bioreactor culture system, a specific cell that is returnedfrom the centrifuge to the bioreactor could be transferred from the bioreactor to the centrifugeagain in the next cycle. These repetitive damages, caused by shear stress from the pump headand centrifugal force, as well as exposure to suboptimal conditions such as oxygen limitationand low temperature below 37C, were more serious at higher perfusion rates. Subsequently,damaged cells and dead cells were continuously accumulated in the bioreactor. Culturetemperature shift from 37 to 33C increased antibody concentrations but showed inhibitory effectson cell growth. The negative effects of lowering culture temperature on cell growth overwhelmedthe positive effects on antibody production. To protect cells from shear stress, Pluronic F-68was 2-fold concentrated in the culture medium; nevertheless, a significantly higher concentrationof Pluronic F-68 (2 g/L) may have inhibitory effects on cell growth.