Identifying Merozoite Surface Protein 4 and Merozoite Surface Protein 7 Plasmodium falciparum Protein Family Members Specifically Binding to Human Erythrocytes Suggests a New Malarial Pa

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• 作者：Yesid Garcia ; Alvaro Puentes ; Hernando Curtidor ; Gladys Cifuentes ; Claudia Reyes ; Jose Barreto ; Armando Moreno ; Manuel E. Patarroyo
• 刊名：Journal of Medicinal Chemistry
• 出版年：2007
• 出版时间：November 15, 2007
• 年：2007
• 卷：50
• 期：23
• 页码：5665 - 5675
• 全文大小：634K
• 年卷期：v.50,no.23(November 15, 2007)
• ISSN：1520-4804

文摘

Plasmodium falciparum merozoite surface proteins (MSP-1 to -11) have been involved in merozoite interactionwith the red blood cell (RBC) surface. Peptides covering complete MSP-4 and MSP-7 amino acid sequenceswere synthesized and tested in RBC binding assays. One MSP-4 high activity binding peptide (HABP) andfive MSP-7 HABPs were found having specific binding to RBC surface. MSP-4 and MSP-7 HABP bindingwas sensitive to enzymatic treatment; they recognized a 52 kDa erythrocyte membrane protein. MSP-4HABP had low invasion inhibition, suggesting it might bind to RBCs and also be involved in physiologicalmechanisms, while MSP-7 HABPs displayed different invasion inhibition activity (83-24%) in in vitrotests, suggesting different roles for both proteins during invasion. Structural characteristics found whencomparing the MSP-4 HABP with MSP-HABPs displaying epidermal growth factor-like sequences suggestedthat these redundant MSP-family proteins could be a new parasite strategy for evading host genetic variabilityand immune pressure.