Proteomic Approach for Caudal Trauma-Induced Acute Phase Proteins Reveals That Creatine Kinase Is a Key Acute Phase Protein in Amphioxus Humoral Fluid

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• 作者：Yuan-yuan Gao ; Dan-feng Zhang ; Hui Li ; Runzhong Liu ; Zheng-hong Zhuang ; Qi-fu Li ; San-ying Wang ; Xuan-xian Peng
• 刊名：Journal of Proteome Research
• 出版年：2007
• 出版时间：November 2007
• 年：2007
• 卷：6
• 期：11
• 页码：4321 - 4329
• 全文大小：579K
• 年卷期：v.6,no.11(November 2007)
• ISSN：1535-3907

文摘

Elevated creatine kinase (CK) in the circulation was generally regarded to be a passive release frommuscle damage. We utilized proteomic methodologies to characterize amphioxus humoral fluid APPsin response to caudal trauma, and found several spots of CK alterations with up-regulation and pIshift. Its amount and enzyme activity showed a dynamic pattern of APP in humoral fluid companiedwith a reduction in enzyme activity of muscle, whereas there was no significant difference in CK amountof muscle and the other tissues and in CK enzyme activity of the other tissues between different timepoints of sample collection following caudal trauma. In addition, CK phosphorylation regulation duringinjury was not achieved by monoclonal antibodies separately against phosphothreonine, phosphotyrosine, and phosphoserine. These results suggested that the CK elevation of humoral fluid might befrom muscle, being an active response to caudal trauma rather than a passive release from muscledamage. Therefore, CK ability in response to caudal trauma should be highly concerned.