文摘
One of the most highlighted and fastest moving interfaces of nanotechnology is the application of quantumdots (QDs) in biology. The unparalleled advantages of the size-tunable fluorescent emission and thesimultaneous excitation at a single wavelength make QDs the great possibility for use in optical encodingdetection. In this paper, we report that green and orange CdTe QDs as convenient, cheap, reversible, andeffective pH-sensitive fluorescent probes could monitor the proton (H+) flux driven by ATP synthesis fordual simultaneous and independent detection of viruses on the basis of antibody-antigen reactions. A newkind of biosensor (consisting of the mixture of green-QDs-labeled chromatophores and orange-QDs-labeledchromatophores) fluorescent measurement system was established for rapid, simultaneous, and independentdetection of two different kinds of viruses (i.e., H9 avian influenza virus and MHV68 virus). It is crucial tofind that the green and orange QDs labeled biosensors coexisting in the detection system can work independentlyand do not interfere with each another in the fluorescence assays. In addition, a primary steady electric doublelayer (EDL) model for the QDs biosensors was proposed to illustrate the mechanism of simultaneous andindependent detection of the biosensors. We believe that the pH-sensitive CdTe QDs based detection system,described in this paper, is an important step toward optical encoding and has a great potential for simultaneousand independent qualitative and quantitative multiple detection systems.