Determination of Free Calcium and Calcium-Containing Species in Human Plasma by Capillary Electrophoresis-Inductively Coupled Plasma Optical Emission Spectrometry

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• 作者：Biyang Deng ; Pingchuan Zhu ; Yingzi Wang ; Jinrong Feng ; Xianfeng Li ; Xiangshu Xu ; Hua Lu ; Qiumei Xu
• 刊名：Analytical Chemistry
• 出版年：2008
• 出版时间：August 1, 2008
• 年：2008
• 卷：80
• 期：15
• 页码：5721-5726
• 全文大小：199K
• 年卷期：v.80,no.15(August 1, 2008)
• ISSN：1520-6882

文摘

A new method for the determination of free calcium concentration in human plasma was developed by online coupling capillary electrophoresis (CE) with inductively coupled plasma optical emission spectrometry (ICP-OES). Baseline separation of calcium-containing species was achieved by CE-ICP-OES in a 120-cm-long capillary with 100-μm internal diameter, at 20 kV applied voltage, with a 30 mmol/L Tris-HCl buffer at pH 7.4. A total of eight calcium-containing species were found in human plasma; the concentration of free calcium ion was found to be 41.9 mg/L. The concentrations of calcium for other seven calcium species, estimated from the calibration against Ca²⁺ standard, were 3.14−15.6 mg/L. The precision (RSD, n = 10) ranged from 1.2 to 2.7% for the migration time and 2.8 to 3.9% for the peak area. The developed method was also applied to analyze plasma samples with recovery ranged from 94.5 to 102% for samples spiked with 40 mg/L free Ca²⁺ ion.