Modularly Assembled Magnetite Nanoparticles Enhance in Vivo Targeting for Magnetic Resonance Cancer Imaging

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• 作者：Ping-Ching Wu ; Chia-Hao Su ; Fong-Yu Cheng ; Jun-Cheng Weng ; Jyh-Horng Chen ; Tsung-Lin Tsai ; Chen-Sheng Yeh ; Wu-Chou Su ; Jih Ru Hwu ; Yonhua Tzeng ; Dar-Bin Shieh
• 刊名：Bioconjugate Chemistry
• 出版年：2008
• 出版时间：October 15, 2008
• 年：2008
• 卷：19
• 期：10
• 页码：1972-1979
• 全文大小：354K
• 年卷期：v.19,no.10(October 15, 2008)
• ISSN：1520-4812

文摘

Modularly assembled targeting nanoparticles were synthesized through self-assembly of targeting moieties on surfaces of functional nanoparticles. Specific molecular recognition of nickel nitrilotriacetate on Fe₃O₄ nanoparticles with hexahistidine tag on RGD4C peptides results in precisely controlled orientation of the targeting peptides. Better selectivity of the self-assembled RGD4C-Fe₃O₄ nanoparticles targeting oral cancer cells than that achievable through a conventional chemical cross-link strategy was demonstrated by means of atomic absorption spectrometry (AAS). An oral cancer hamster model was applied to reveal specific in vivo targeting and MR molecular imaging contrast in cancer lesions expressing αvβ3 integrin. Both AAS and MRI revealed that the self-assembled nanoparticles improved the targeting efficiency and reduced the hepatic uptake as compared with the conventional chemical cross-link particles. We investigated the biosafety, biodistribution, and kinetics of the nanoparticles and found that the nanoparticles were significantly cleared from the liver and kidneys after one week. By recombining the desired targeting moiety and various functional nanoparticles through self-assembly, this new modularly designed platform has the capability of enhancing the efficiency of targeted diagnosis and therapies for a wide spectrum of biomedical applications.