Conditional Glycosylation in Eukaryotic Cells Using a Biocompatible Chemical Inducer of Dimerization

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• 作者：Jennifer L. Czlapinski ; Michael W. Schelle ; Lawrence W. Miller ; Scott T. Laughlin ; Jennifer J. Kohler ; Virginia W. Cornish ; Carolyn R. Bertozzi
• 刊名：Journal of the American Chemical Society
• 出版年：2008
• 出版时间：October 8, 2008
• 年：2008
• 卷：130
• 期：40
• 页码：13186-13187
• 全文大小：157K
• 年卷期：v.130,no.40(October 8, 2008)
• ISSN：1520-5126

文摘

Chemical inducers of dimerization (CIDs) are cell-permeable small molecules capable of dimerizing two protein targets. The most widely used CID, the natural product rapamycin and its relatives, is immunosuppressive due to interactions with endogenous targets and thus has limited utility in vivo. Here we report a new biocompatible CID, Tmp-SLF, which dimerizes E. coli DHFR and FKBP and has no endogenous mammalian targets that would lead to unwanted in vivo side effects. We employed Tmp-SLF to modulate gene expression in a yeast three-hybrid assay. Finally, we engineered the Golgi-resident glycosyltransferase FucT7 for tunable control by Tmp-SLF in mammalian cells.