Synthesis of DNA−Organic Molecule−DNA Triblock Oligomers Using the Amide Coupling Reaction and Their Enzymatic Amplification

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• 作者：Jungkyu K. Lee ; Young Hwan Jung ; Randall M. Stoltenberg ; Jeffery B.-H. Tok ; Zhenan Bao
• 刊名：Journal of the American Chemical Society
• 出版年：2008
• 出版时间：October 1, 2008
• 年：2008
• 卷：130
• 期：39
• 页码：12854-12855
• 全文大小：128K
• 年卷期：v.130,no.39(October 1, 2008)
• ISSN：1520-5126

文摘

Precise electrical contact between single-molecule and electrodes is a first step to study single-molecule electronics and its application such as (bio)sensors and nanodevices. To realize a reliable electrical contact, we can use DNA as a template in the field of nanoelectronics because of its micrometer-scaled length with the thickness of nanometer-scale. In this paper, we studied the reactivity of the amide-coupling reaction to tether oligodeoxynucleotides (ODNs) to organic molecules and the elongation of the ODNs by the polymerase chain reaction (PCR) to synthesize 1.5 kbp dsDNA-organic molecule-1.5 kbp dsDNA (DOD) triblock architecture. The successful amide-coupling reactions were confirmed by electrospray ionization mass spectrometry (ESI-MS), and the triblock architectures were characterized by 1% agarose gel electrophoresis and atomic force microscope (AFM). Our result shows that this strategy is simple and makes it easy to construct DNA−organic molecule−DNA triblock architectures and potentially provides a platform to prepare and investigate single molecule electronics.