Isolation, Structure, and Antibacterial Activity of Phaeosphenone from a Phaeosphaeria sp. Discovered by Antisense Strategy

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• 作者：Chaowei Zhang ; John G. Ondeyka ; Deborah L. Zink ; Angela Basilio ; Francisca Vicente ; Javier Collado ; Gonzalo Platas ; Gerald Bills ; Joann Huber ; Karen Dorso ; Mary Motyl ; Kevin Byrne ; Sheo B. Singh
• 刊名：Journal of Natural Products
• 出版年：2008
• 出版时间：July 2008
• 年：2008
• 卷：71
• 期：7
• 页码：1304-1307
• 全文大小：125K
• 年卷期：v.71,no.7(July 2008)
• ISSN：1520-6025

文摘

Ribosomal protein S4 (RPSD), a part of the ribosomal small subunit, is one of the proteins that is a part of the ribosomal machinery and is a potential new target for the discovery of antibacterial agents. Continued screening of microbial extracts using antisense-sensitized rpsD Staphylococcus aureus strain led to the isolation of a new dimeric compound, phaeosphenone (2). Compound 2 showed broad-spectrum antibacterial activity against Gram-positive bacteria, exhibiting MIC values ranging from 8 to 64 μg/mL. Phaeosphenone showed the highest sensitivity for Streptococcus pneumoniae (8 μg/mL) and inhibited the growth of Candida albicans with an MIC of 8 μg/mL. Phaeosphenone showed a modest selectivity for the inhibition of RNA synthesis over DNA and protein synthesis in S. aureus.