Characterization and Antioxidant Activity of Essential Oils from Fresh and Decaying Leaves of Eucalyptus tereticornis

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• 作者：Harminder P. Singh ; Sunil Mittal ; Shalinder Kaur ; Daizy R. Batish ; Ravinder K. Kohli
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2009
• 出版时间：August 12, 2009
• 年：2009
• 卷：57
• 期：15
• 页码：6962-6966
• 全文大小：698K
• 年卷期：v.57,no.15(August 12, 2009)
• ISSN：1520-5118

文摘

The composition of essential oils hydrodistilled from fresh and decaying leaves of Eucalyptus tereticornis was analyzed by means of gas chromatography and mass spectrometry, and a total of 68 constituents were identified. The essential oils were assayed for antioxidant activity in terms of scavenging of 2,2-diphenyl-1-picrylhydrazil (DPPH) and hydroxyl (OH^•) radical, and superoxide anion (O₂^−•).The major constituents of the fresh leaf oil were α-pinene (28.53%) and 1,8-cineole (19.48%), whereas in the decaying leaf oil, β-citronellal (14.15%), (−)-isopulegol (13.35%), and (+)-β-citronellol (10.73%) were the major components. Both essential oils exhibited a strong radical scavenging activity against DPPH radical with IC₅₀ values of 110 and 139.8 μg/mL for fresh and decaying leaf oil, respectively (IC₅₀ of BHT = 164.2 μg/mL). Further, the essential oils (at 400 μg/mL) also exhibited OH^• (56−62%) and O₂^−• (65−69%) scavenging activity parallel to the commercial antioxidant BHT/ascorbic acid. However, unlike the essential oils, the major monoterpene constituents exhibited significantly less scavenging activity (<35% DPPH or OH^•; at 400 μg/mL). The study concluded that fresh and decaying leaves of E. tereticornis are a source of monoterpenoid rich oil exhibiting antioxidant activity.