A Novel Type of Dual-Modality Molecular Probe for MR and Nuclear Imaging of Tumor: Preparation, Characterization and in Vivo Application

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• 作者：Shujie Liu ; Bing Jia ; Ruirui Qiao ; Zhi Yang ; Zilin Yu ; Zhaofei Liu ; Kan Liu ; Jiyun Shi ; Han Ouyang ; Fan Wang ; Mingyuan Gao
• 刊名：Molecular Pharmaceutics
• 出版年：2009
• 出版时间：August 3, 2009
• 年：2009
• 卷：6
• 期：4
• 页码：1074-1082
• 全文大小：288K
• 年卷期：v.6,no.4(August 3, 2009)
• ISSN：1543-8392

文摘

A novel dual-modality molecular probe composed of biocompatible Fe₃O₄ nanocrystal, monoclonal antibody and radionuclide was designed and prepared. All functional components in the dual-modality molecular probe, i.e., Fe₃O₄, PEG, mAb 3H11 and ¹²⁵I, were chemically bonded together for forming a stable molecular probe. Systematic in vitro experiments were carried out for evaluating the biological activity of the antibody in the targeting probe. A series of in vivo experiments were performed based on the dual-modality imaging probe for detecting xenografted tumors in nude mice by MRI and γ-imaging techniques. The pharmacokinetics of the dual-modality molecular probe in tumor-bearing nude mice was studied.