Semisynthetic Tellurosubtilisin with Glutathione Peroxidase Activity

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• 作者：Shizhong Mao ; Zeyuan Dong ; Junqiu Liu ; Xiangqiu Li ; Xiaoman Liu ; Guimin Luo ; and Jiacong Shen
• 刊名：Journal of the American Chemical Society
• 出版年：2005
• 出版时间：August 24, 2005
• 年：2005
• 卷：127
• 期：33
• 页码：11588 - 11589
• 全文大小：44K
• 年卷期：v.127,no.33(August 24, 2005)
• ISSN：1520-5126

文摘

Reaction of the hydroxyl group of serine-221 of subtilisin with phenylmethanesulfonylfluoride followed by nucleophilic substitution with sodium hydrogen telluride, a semisynthetic telluroprotein, tellurosubtilisin, was prepared. Tellurosubtilisin, which displays high substrate specificity for aromatic thiols, exhibits remarkable peroxidase activity and catalyzes the reduction of hydrogen peroxide by 3-carboxy-4-nitrobenzenethiol 20 000 times more efficiently than diphenyl diselenide.