A High-Throughput Multiplex Method Adapted for GMO Detection

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• 作者：Maher Chaouachi ; Galle Chupeau ; Aurlie Berard ; Heather McKhann ; Marcel Romaniuk ; Sandra Giancola ; Valrie Laval ; Yves Bertheau ; Dominique Brunel
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2008
• 出版时间：December 24, 2008
• 年：2008
• 卷：56
• 期：24
• 页码：11596-11606
• 全文大小：507K
• 年卷期：v.56,no.24(December 24, 2008)
• ISSN：1520-5118

文摘

A high-throughput multiplex assay for the detection of genetically modified organisms (GMO) was developed on the basis of the existing SNPlex method designed for SNP genotyping. This SNPlex assay allows the simultaneous detection of up to 48 short DNA sequences (70 bp; “signature sequences”) from taxa endogenous reference genes, from GMO constructions, screening targets, construct-specific, and event-specific targets, and finally from donor organisms. This assay avoids certain shortcomings of multiplex PCR-based methods already in widespread use for GMO detection. The assay demonstrated high specificity and sensitivity. The results suggest that this assay is reliable, flexible, and cost- and time-effective for high-throughput GMO detection.