Identification of a Set of Genes Involved in the Formation of the Substrate for the Incorporation of the Unusual "Glycolate" Chain Extension Unit in Ansamitocin Biosynthesis

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• 作者：Brian J. Carroll ; Steven J. Moss ; Linquan Bai ; Yasuo Kato ; Sabine Toelzer ; Tin-Wein Yu ; and Heinz G. Floss
• 刊名：Journal of the American Chemical Society
• 出版年：2002
• 出版时间：April 24, 2002
• 年：2002
• 卷：124
• 期：16
• 页码：4176 - 4177
• 全文大小：45K
• 年卷期：v.124,no.16(April 24, 2002)
• ISSN：1520-5126

文摘

The unusual "glycolate" extender unit at C-9/C-10 of ansamitocin is not derived from 2-hydroxymalonyl-CoA or 2-methoxymalonyl-CoA, as demonstrated by feeding experiments with the corresponding 1-¹³C-labeled N-acetylcysteamine thioesters but is formed from an acyl carrier protein (ACP)-bound substrate, possibly 2-methoxymalonyl-ACP, elaborated by enzymes encoded by a subcluster of five genes, asm12-17, from the ansamitocin bisosynthetic gene cluster.