Polishable and Renewable DNA Hybridization Biosensors

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• 作者：Joseph Wang ; Joã ; o Roberto Fernandes ; and Lauro Tatsuo Kubota
• 刊名：Analytical Chemistry
• 出版年：1998
• 出版时间：September 1, 1998
• 年：1998
• 卷：70
• 期：17
• 页码：3699 - 3702
• 全文大小：48K
• 年卷期：v.70,no.17(September 1, 1998)
• ISSN：1520-6882

文摘

Routine applications of DNA hybridization biosensors areoften restricted by the need for regenerating the single-stranded (ss) probe for subsequent reuse. This notereports on a viable alternative to prolonged thermal orchemical regeneration schemes through the mechanicalpolishing of oligonucleotide-bulk-modified carbon composite electrodes. The surface of these biocompositehybridization biosensors can be renewed rapidly andreproducibly by a simple extrusion/polishing protocol.The immobilized probe retains its hybridization activityon confinement in the interior of the carbon paste matrix,with the use of fresh surfaces erasing memory effects andrestoring the original target response, to allow numeroushybridization/measurement cycles. We expect that suchreusable nucleic acid modified composite electrodes canbe designed for a wide variety of biosensing applications.