Spatially Controlled Photochemical Peptide and Polymer Conjugation on Biosurfaces

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• 作者：Thomas Tischer ; Tanja K. Claus ; Michael Bruns ; Vanessa Trouillet ; Katharina Linkert ; Cesar Rodriguez-Emmenegger ; Anja S. Goldmann ; S茅bastien Perrier ; Hans G. B枚rner ; Christopher Barner-Kowollik
• 刊名：Biomacromolecules
• 出版年：2013
• 出版时间：December 9, 2013
• 年：2013
• 卷：14
• 期：12
• 页码：4340-4350
• 全文大小：606K
• 年卷期：v.14,no.12(December 9, 2013)
• ISSN：1526-4602

文摘

An efficient phototriggered Diels鈥揂lder conjugation is utilized to graft in an effective and straightforward approach poly(trifluoro ethyl methacrylate) (M_n = 3700 Da, 膼 = 1.27) and a model peptide (GIGKFLHS) onto thin hyaluronan films and cellulose surfaces. The surfaces were functionalized with an o-quinodimethane moiety 鈥?capable of releasing a caged diene 鈥?via carbodiimide mediated coupling. The o-quinodimethane group is employed as a photoactive linker to tether predefined peptide/polymer strands in a spatially controlled manner onto the biosurface by photoenol ligation. An in-depth characterization employing XPS, ToF-SIMS, SPR, ellipsometry, and AFM was conducted to evidence the effectiveness of the presented approach.