Separation and Identification of Peptides in Single Neurons by Microcolumn Liquid Chromatography-Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry and Postsource Decay Analy

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• 作者：Showchien Hsieh ; Klaus Dreisewerd ; Roel C. van der Schors ; Connie R. Jimé ; nez ; Jianru Stahl-Zeng ; Franz Hillenkamp ; James W. Jorgenson ; Wijnand P. M. Geraerts ; and Ka Wan Li
• 刊名：Analytical Chemistry
• 出版年：1998
• 出版时间：May 1, 1998
• 年：1998
• 卷：70
• 期：9
• 页码：1847 - 1852
• 全文大小：93K
• 年卷期：v.70,no.9(May 1, 1998)
• ISSN：1520-6882

文摘

Microcolumn liquid chromatography (LC) was interfacedwith matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) for separationand identification of peptides present in single neuronsfrom the brain of the snail Lymnaea stagnalis.Thenanoliter microcolumn LC effluent, mixed off-line withnanoliter matrix solution, was deposited onto the sampletarget every 60 s, producing fractions of approximately145 nL in volume, which, upon drying, produced spotsof approximately 1 mm in size. At the end of thechromatographic separation, fractions from the sampletarget were scanned by MALDI-TOF-MS. Identificationof peptide peaks was achieved on the basis of LC elutionorder and mass information. Further identificationbasedon sequence information was carried out for a nativepeptide fractionated by microcolumn LC from a singleneuron with the postsource decay technique.