Effects of Atmospheric Ozone on Microarray Data Quality

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• 作者：Thomas L. Fare ; Ernest M. Coffey ; Hongyue Dai ; Yudong D. He ; Deborah A. Kessler ; Kristopher A. Kilian ; John E. Koch ; Eric LeProust ; Matthew J. Marton ; Michael R. Meyer ; Roland B. Stoughton ; George Y.
• 刊名：Analytical Chemistry
• 出版年：2003
• 出版时间：September 1, 2003
• 年：2003
• 卷：75
• 期：17
• 页码：4672 - 4675
• 全文大小：208K
• 年卷期：v.75,no.17(September 1, 2003)
• ISSN：1520-6882

文摘

A data anomaly was observed that affected the uniformityand reproducibility of fluorescent signal across DNAmicroarrays. Results from experimental sets designed toidentify potential causes (from microarray production toarray scanning) indicated that the anomaly was linked toa batch process; further work allowed us to localize theeffect to the posthybridization array stringency washes.Ozone levels were monitored and highly correlated withthe batch effect. Controlled exposures of microarrays toozone confirmed this factor as the root cause, and wepresent data that show susceptibility of a class of cyaninedyes (e.g., Cy5, Alexa 647) to ozone levels as low as 5-10ppb for periods as short as 10-30 s. Other cyanine dyes(e.g., Cy3, Alexa 555) were not significantly affected untilhigher ozone levels (>100 ppb). To address this environmental effect, laboratory ozone levels should be kept below2 ppb (e.g., with filters in HVAC) to achieve high qualitymicroarray data.