Comparison of Isotope Dilution Mass Spectrometry Methods for the Determination of Total Homocysteine in Plasma and Serum

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• 作者：Mary B. Satterfield ; Lorna T. Sniegoski ; Michael J. Welch ; Bryant C. Nelson ; and Christine M. Pfeiffer
• 刊名：Analytical Chemistry
• 出版年：2003
• 出版时间：September 1, 2003
• 年：2003
• 卷：75
• 期：17
• 页码：4631 - 4638
• 全文大小：108K
• 年卷期：v.75,no.17(September 1, 2003)
• ISSN：1520-6882

文摘

Two independent methods have been critically evaluatedand applied to the measurement of total homocysteine inserum and plasma: solid-phase anion extraction (SPAE)gas chromatography/mass spectrometry (GC/MS) andprotein precipitation liquid chromatography/tandem massspectrometry (LC/MS/MS). In addition, analysis of samplesprepared by SPAE was accomplished by liquid chromatography/mass spectrometry (LC/MS) and LC/MS/MS.These methods have been used to determine total homocysteine levels in several existing serum-based Standard Reference Materials (SRMs) from the NationalInstitute of Standards and Technology and in patientplasma samples provided by the Centers for DiseaseControl and Prevention. The precision of the homocysteine measurements in serum and plasma was criticallyevaluated, and method comparisons were carried outusing Bland-Altman plots and bias analysis. On the basisof the excellent precision and close agreement of the massspectrometric (MS) methods, the MS-based methods willbe used for certification of a serum-based SRM forhomocysteine and folates.