Fabrication of Integrated Microelectrodes for Electrochemical Detection on Electrophoresis Microchip by Electroless Deposition and Micromolding in Capillary Technique

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• 作者：Jilin Yan ; Yan Du ; Jifeng Liu ; Weidong Cao ; Xiuhua Sun ; Weihong Zhou ; Xiurong Yang ; and ; and Erkang Wang
• 刊名：Analytical Chemistry
• 出版年：2003
• 出版时间：October 15, 2003
• 年：2003
• 卷：75
• 期：20
• 页码：5406 - 5412
• 全文大小：168K
• 年卷期：v.75,no.20(October 15, 2003)
• ISSN：1520-6882

文摘

A new method for the fabrication of an integrated microelectrode for electrochemical detection (ECD) on anelectrophoresis microchip is described. The pattern of themicroelectrode was directly made on the surface of amicroscope slide through an electroless deposition procedure. The surface of the slide was first selectively coatedwith a thin layer of sodium silicate through a micromolding in capillary technique provided by a poly(dimethylsiloxane) (PDMS) microchannel; this left a rough patternedarea for the anchoring of catalytic particles. A metal layerwas deposited on the pattern guided by these catalyticparticles and was used as the working electrode. Factorsinfluencing the fabrication procedure were discussed. Thewhole chip was built by reversibly sealing the slide toanother PDMS layer with electrophoresis microchannelsat room temperature. This approach eliminates the needof clean room facilities and expensive apparatus such asfor vacuum deposition or sputtering and makes it possibleto produce patterned electrodes suitable for ECD onmicrochip under ordinary chemistry laboratory conditions. Also once the micropattern is ready, it allows theresearchers to rebuild the electrode in a short period oftime when an electrode failure occurs. Copper and goldmicroelectrodes were fabricated by this technique. Glucose, dopamine, and catechol as model analytes weretested.