Improved Mass Analysis of Oligoribonucleotides by 13C, 15N Double Depletion and Electrospray Ionization FT-ICR Mass Spectrometry

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• 作者：Ying Xiong ; Kersten Schroeder ; Nancy L. Greenbaum ; Christopher L. Hendrickson ; and Alan G. Marshall
• 刊名：Analytical Chemistry
• 出版年：2004
• 出版时间：March 15, 2004
• 年：2004
• 卷：76
• 期：6
• 页码：1804 - 1809
• 全文大小：91K
• 年卷期：v.76,no.6(March 15, 2004)
• ISSN：1520-6882

文摘

¹³C, ¹⁵N doubly depleted 32-ribonucleotide was synthesized enzymatically by in vitro transcription from nucleoside triphosphates isolated from E. coli grown in aminimal medium containing ¹²C, ¹⁴N-enriched glucoseand ammonium sulfate. Following purification and desalting by reversed-phase HPLC, buffer exchange withMicrocon YM-3, and ethanol precipitation, electrosprayionization Fourier transform ion cyclotron resonance massspectra revealed greatly enhanced abundance of monoisotopic ions (by a factor of ~100) and a narrower isotopicdistribution with higher signal-to-noise ratio. The abruptonset and high magnitude of the monoisotopic speciespromise to facilitate accurate mass measurement ofRNA's.