Microchip Capillary Electrophoresis with an Integrated Indium Tin Oxide Electrode-Based Electrochemiluminescence Detector

详细信息    查看全文

• 作者：Haibo Qiu ; Jilin Yan ; Xiuhua Sun ; Jifeng Liu ; Weidong Cao ; Xiurong Yang ; and Erkang Wang
• 刊名：Analytical Chemistry
• 出版年：2003
• 出版时间：October 15, 2003
• 年：2003
• 卷：75
• 期：20
• 页码：5435 - 5440
• 全文大小：110K
• 年卷期：v.75,no.20(October 15, 2003)
• ISSN：1520-6882

文摘

This paper describes an indium tin oxide (ITO) electrode-based Ru(bpy)₃²⁺ electrochemiluminecence (ECL) detector for a microchip capillary electrophoresis (CE). Themicrochip CE-ECL system described in this article consists of a poly(dimethylsiloxane) (PDMS) layer containingseparation and injection channels and an electrode platewith an ITO electrode fabricated by a photolithographicmethod. The PDMS layer was reversibly bound to the ITOelectrode plate, which greatly simplified the alignment ofthe separation channel with the working electrode andenhanced the photon-capturing efficiency. In our study,the high separation electric field had no significant influence on the ECL detector, and decouplers for isolatingthe separation electric field were not needed in themicrochip CE-ECL system. The ITO electrodes employedin the experiments displayed good durability and stabilityin the analytical procedures. Proline was selected toperform the microchip device with a limit of detection of1.2 M (S/N = 3) and a linear range from 5 to 600 M.