Superresolution Imaging of Targeted Proteins in Fixed and Living Cells Using Photoactivatable Organic Fluorophores

详细信息    查看全文

• 作者：Hsiao-lu D. Lee ; Samuel J. Lord ; Shigeki Iwanaga ; Ke Zhan ; Hexin Xie ; Jarrod C. Williams ; Hui Wang ; Grant R. Bowman ; Erin D. Goley ; Lucy Shapiro ; Robert J. Twieg ; Jianghong Rao ; W. E. Moerner
• 刊名：Journal of the American Chemical Society
• 出版年：2010
• 出版时间：November 3, 2010
• 年：2010
• 卷：132
• 期：43
• 页码：15099-15101
• 全文大小：197K
• 年卷期：v.132,no.43(November 3, 2010)
• ISSN：1520-5126

文摘

Superresolution imaging techniques based on sequential imaging of sparse subsets of single molecules require fluorophores whose emission can be photoactivated or photoswitched. Because typical organic fluorophores can emit significantly more photons than average fluorescent proteins, organic fluorophores have a potential advantage in super-resolution imaging schemes, but targeting to specific cellular proteins must be provided. We report the design and application of HaloTag-based target-specific azido DCDHFs, a class of photoactivatable push−pull fluorogens which produce bright fluorescent labels suitable for single-molecule superresolution imaging in live bacterial and fixed mammalian cells.