Distribution and in Vitro Availability of Selenium in Selenium-Containing Storage Protein from Selenium-Enriched Rice Utilizing Optimized Extraction

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• 作者：Yong Fang ; Brittany Catron ; Yaofang Zhang ; Liyan Zhao ; Joseph a. Caruso ; Qiuhui Hu
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2010
• 出版时间：September 8, 2010
• 年：2010
• 卷：58
• 期：17
• 页码：9731-9738
• 全文大小：860K
• 年卷期：v.58,no.17(September 8, 2010)
• ISSN：1520-5118

文摘

Selenium (Se) distribution in Se-enriched rice and optimization of extraction for Se-containing protein were studied. Se availability in Se-containing protein product was simulated using an in vitro gastrointestinal digestion. The results showed that Se was predominately found as organic Se, whereas inorganic Se comprised only 2.85% of the total Se. The glutelin fraction contained the largest amount of Se, approximately 31.3% of the total Se in the rice gain. Utilizing orthogonal analysis, the optimum extraction conditions were selected at a volume to weight of 20:1, 0.08 M NaOH, an extraction time of 3 h, and at a temperature of 35 °C. A Se-containing rice protein product with 83.5% protein and 9.09 μg g⁻¹ Se was sequestered using the optimal extraction method. This rice protein product with high molecular weight Se-containing protein can readily be digested to low molecular weight peptides and selenomethionine (52.3% of total Se in protein extract).

Keywords:

Selenium; rice protein; distribution; availability; extraction