Proteomic Analysis of Interstitial Fluid in Bone Marrow Identified That Peroxiredoxin 2 Regulates H2O2 Level of Bone Marrow during Aging

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• 作者：Wei Wang ; Lantu Gou ; Gang Xie ; Aiping Tong ; Fei He ; Zejun Lu ; Yuqin Yao ; Kang Liu ; Jie Li ; Minghai Tang ; Lijuan Chen ; Jinliang Yang ; Huozhen Hu ; Yu-Quan Wei
• 刊名：Journal of Proteome Research
• 出版年：2010
• 出版时间：August 6, 2010
• 年：2010
• 卷：9
• 期：8
• 页码：3812-3819
• 全文大小：314K
• 年卷期：v.9,no.8(August 6, 2010)
• ISSN：1535-3907

文摘

Hematopoiesis in bone marrow declines during aging owing to alteration of the hematopoietic niche. However, due to difficult accessibility and other complexities, senescence-related alteration of the hematopoietic niche is largely unknown. The interstitial fluid of bone marrow (IFBM), a pivotal component of the hematopoietic niche, includes soluble secretory factors that are present between bone marrow cells. To characterize the proteomic profile changes of IFBM during aging, we analyzed the IFBMs of young, adult, and senescent rats using 2-DE combined with ESI/MALDI-Q-TOF MS. Finally, 31 differentially expressed proteins involved in multiple biological functions were identified. Peroxiredoxin 2 (Prx2), down-regulated during aging, was further analyzed and demonstrated that it is produced by bone marrow stromal cells. Interestingly, higher levels of hydrogen peroxide (H₂O₂) were detected in the bone marrow with lower Prx2 expression. Moreover, exogenous Prx2 reduced the intracellular H₂O₂ level in bone marrow stromal cells in vitro. Therefore, Prx2 is implied in the regulation of H₂O₂ production in the bone marrow during aging. Our data characterized the dynamic protein profiles of the bone marrow microenvironment during aging and we provided clues to elucidate the mechanism of creating a low ROS level in the hematopoietic niche.

Keywords:

Aging; Proteomics; Hematopoiesis; Bone marrow; Peroxiredoxin 2; H₂O₂; Niche