A Safe and Practical Procedure for Global Deprotection of Oligoribonucleotides

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• 作者：Daniel Zewge ; Francis Gosselin ; Rick Sidler ; Lisa DiMichele ; Raymond J. Cvetovich
• 刊名：Journal of Organic Chemistry
• 出版年：2010
• 出版时间：August 6, 2010
• 年：2010
• 卷：75
• 期：15
• 页码：5305-5307
• 全文大小：740K
• 年卷期：v.75,no.15(August 6, 2010)
• ISSN：1520-6904

文摘

We report a practical global deprotection of RNA 2′-O-tert-butyldimethylsilyl (TBS) ethers using commercially available aqueous NH₄F. The procedure is applicable to both 96-well plate format and large-scale production of RNA. This improved procedure provides a safe, mild, and cost-effective alternative to highly hazardous Et₃N·3HF, a reagent commonly used in the routine synthesis of RNA.