Structural Analysis of Protein Interfaces from 13C Direct-Detected Paramagnetic Relaxation Enhancements

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• 作者：Tobias Madl ; Isabella C. Felli ; Ivano Bertini ; Michael Sattler
• 刊名：Journal of the American Chemical Society
• 出版年：2010
• 出版时间：June 2, 2010
• 年：2010
• 卷：132
• 期：21
• 页码：7285-7287
• 全文大小：213K
• 年卷期：v.132,no.21(June 2, 2010)
• ISSN：1520-5126

文摘

The measurement of ¹³C directed-detected paramagnetic relaxation enhancements (PREs) on spin-labeled proteins combines the efficacy of PREs for the detection of long-range distance information with the favorable sensitivity and resolution of ¹³C direct-detected experiments. The ¹³C PREs provide long-range distance restraints to map binding interfaces in proteins and protein complexes and are especially useful for studies of high-molecular weight perdeuterated molecules.