Polyamidoamine-Grafted Multiwalled Carbon Nanotubes for Gene Delivery: Synthesis, Transfection and Intracellular Trafficking

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• 作者：Min Liu ; Biao Chen ; Yanan Xue ; Jie Huang ; Liming Zhang ; Shiwen Huang ; Qingwen Li ; Zhijun Zhang
• 刊名：Bioconjugate Chemistry
• 出版年：2011
• 出版时间：November 16, 2011
• 年：2011
• 卷：22
• 期：11
• 页码：2237-2243
• 全文大小：408K
• 年卷期：v.22,no.11(November 16, 2011)
• ISSN：1520-4812

文摘

Functionalized multiwalled carbon nanotubes (f-MWNTs) are of great interest and designed as a novel gene delivery system. In this paper, we presented synthesis of polyamidoamine-functionalized multiwalled carbon nanotubes (PAA-g-MWNTs) and their application as a novel gene delivery system. The PAA-g-MWNTs, obtained from amide formation between PAA and chemically oxidized MWNTs, were stable in aqueous solution and much less toxic to cells than PAA and PEI 25KDa. More importantly, PAA-g-MWNTs showed comparable or even higher transfection efficiency than PAA and PEI at optimal w/w ratio. Intracellular trafficking of Cy3-labeled pGL-3 indicated that a large number of Cy3-labeled pGL-3 were attached to nucleus membrane, the majority of which was localized in nucleus after incubation with cells for 24 h. We have demonstrated that PAA modification of MWNTs facilitate higher DNA uptake and gene expression in vitro. All these facts suggest potential application of PAA-g-MWNTs as a novel gene vector with high transfection efficiency and low cytotoxicity.