High-Resolution, Two-Dimensional Measurement of Dissolved Reactive Phosphorus in Sediments Using the Diffusive Gradients in Thin Films Technique in Combination with a Routine Procedure

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• 作者：Shiming Ding ; Fei Jia ; Di Xu ; Qin Sun ; Lei Zhang ; Chengxin Fan ; Chaosheng Zhang
• 刊名：Environmental Science & Technology
• 出版年：2011
• 出版时间：November 15, 2011
• 年：2011
• 卷：45
• 期：22
• 页码：9680-9686
• 全文大小：915K
• 年卷期：v.45,no.22(November 15, 2011)
• ISSN：1520-5851

文摘

Dissolved reactive phosphorus (DRP) is the most available P form in sediments and often directly controls phytoplankton blooms in aquatic systems. In this study, a novel procedure was developed for two-dimensional (2D) measurement of DRP in sediments at a spatial resolution of 0.45 mm using the diffusive gradients in thin films (DGT) technique with a revised high-capacity binding phase (Zr oxide gel). This procedure involves DGT uptake of P in sediments, 2D slicing of the binding gel on a 0.45 脳 0.45-mm grid system, elution of P from each gel square with 1 M NaOH, and microcolorimetric determination of DRP in each eluted solution using 384-microwell plates. Measurements of DRP via this procedure were tested in homogeneous solutions and sediments and produced an acceptable error (<20% relative standard deviation) for the analysis once the accumulated mass of P in each gel square reached 1.2 渭g cm^鈥?. This method was successfully applied to produce 2D images of the DRP distribution in sediments with and without the influence of tubificid worm bioturbation, revealing a much more pronounced and localized impact from tubificid worms than that found using a one-dimensional measurement of pore water DRP concentrations at 1-cm resolution.