Ratiometric Phosphorescence Imaging of Hg(II) in Living Cells Based on a Neutral Iridium(III) Complex

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• 作者：Yongquan Wu ; Hao Jing ; Zesheng Dong ; Qiang Zhao ; Huazhou Wu ; Fuyou Li
• 刊名：Inorganic Chemistry
• 出版年：2011
• 出版时间：August 15, 2011
• 年：2011
• 卷：50
• 期：16
• 页码：7412-7420
• 全文大小：1096K
• 年卷期：v.50,no.16(August 15, 2011)
• ISSN：1520-510X

文摘

In this work, a neutral iridium(III) complex [Ir(bt)₂(acac)] (Hbt = 2-phenylbenzothiazole; Hacac = acetylacetone) has been realized as a Hg(II)-selective sensor through UV鈥搗is absorption, phosphorescence emission, and electrochemical measurements and was further developed as a phosphorescent agent for monitoring intracellular Hg(II). Upon addition of Hg(II) to a solution of [Ir(bt)₂(acac)], a noticeable spectral blue shift in both absorption and phosphorescent emission bands was measured. ¹H NMR spectroscopic titration experiments indicated that coordination of Hg(II) to the complex induces fast decomposition of [Ir(bt)₂(acac)] to form a new complex, which is responsible for the significant variations in optical and electrochemical signals. Importantly, cell imaging experiments have shown that [Ir(bt)₂(acac)] is membrane permeable and can be used to monitor the changes in Hg(II) levels within cells in a ratiometric phosphorescence mode.