In Vitro Imaging and Human Serum Albumin Responsive Dimeric Lanthanide DO3A Complex

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• 作者：Yuen On Fung ; Wanqing Wu ; Chi-Tung Yeung ; Hoi-Kuan Kong ; Kenny Kam-Cheng Wong ; Wai-Sum Lo ; Ga-Lai Law ; Ka-Leung Wong ; Chi-Kong Lau ; Chi-Sing Lee ; Wing-Tak Wong
• 刊名：Inorganic Chemistry
• 出版年：2011
• 出版时间：June 20, 2011
• 年：2011
• 卷：50
• 期：12
• 页码：5517-5525
• 全文大小：1090K
• 年卷期：v.50,no.12(June 20, 2011)
• ISSN：1520-510X

文摘

Two series of dimeric DO3A (1,4,7,10-tetraazacyclodecane-1,4,7-triacetate) lanthanide complexes (LnL₁鈥揕nL₂, Ln = Eu, Gd, and Tb) have been synthesized with two different bridged chromophores. The X-ray structures of dimeric LnL₁ (Ln = Gd and Tb) complexes show that each metal ion has nine coordination numbers with eight directly bound donor atoms of the ligand and one oxygen donor from the water molecule. Photophysical measurements indicate that the bridged antenna in LnL₂ gives a higher efficiency than that of LnL₁ and is responsive to the protein Human Serum Albumin (HSA), giving an f鈥揻 luminescence signal enhancement with a binding constant log K = 4.84. In vitro imaging of EuL₁ and EuL₂ in HeLa cells has been recorded, and EuL₂ has demonstrated a higher rate of cellular uptake and low cytotoxicity (IC₅₀ = 3 mM).