Development of a Far-Red to Near-Infrared Fluorescence Probe for Calcium Ion and its Application to Multicolor Neuronal Imaging

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• 作者：Takahiro Egawa ; Kenjiro Hanaoka ; Yuichiro Koide ; Sakiko Ujita ; Naoya Takahashi ; Yuji Ikegaya ; Norio Matsuki ; Takuya Terai ; Tasuku Ueno ; Toru Komatsu ; Tetsuo Nagano
• 刊名：Journal of the American Chemical Society
• 出版年：2011
• 出版时间：September 14, 2011
• 年：2011
• 卷：133
• 期：36
• 页码：14157-14159
• 全文大小：723K
• 年卷期：v.133,no.36(September 14, 2011)
• ISSN：1520-5126

文摘

To improve optical imaging of Ca²⁺ and to make available a distinct color window for multicolor imaging, we designed and synthesized CaSiR-1, a far-red to near-infrared fluorescence probe for Ca²⁺, using Si-rhodamine (SiR) as the fluorophore and the well-known Ca²⁺ chelator BAPTA. This wavelength region is advantageous, affording higher tissue penetration, lower background autofluorescence, and lower phototoxicity in comparison with the UV to visible range. CaSiR-1 has a high fluorescence off/on ratio of over 1000. We demonstrate its usefulness for multicolor fluorescence imaging of action potentials (visualized as increases in intracellular Ca²⁺) in brain slices loaded with sulforhodamine 101 (red color; specific for astrocytes) that were prepared from transgenic mice in which some neurons expressed green fluorescent protein.