Ratiometric Detection of Mitochondrial Thiols with a Two-Photon Fluorescent Probe

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• 作者：Chang Su Lim ; Goutam Masanta ; Hyung Joong Kim ; Ji Hee Han ; Hwan Myung Kim ; Bong Rae Cho
• 刊名：Journal of the American Chemical Society
• 出版年：2011
• 出版时间：July 27, 2011
• 年：2011
• 卷：133
• 期：29
• 页码：11132-11135
• 全文大小：792K
• 年卷期：v.133,no.29(July 27, 2011)
• ISSN：1520-5126

文摘

We report a ratiometric two-photon probe (SSH-Mito) for mitochondrial thiols. This probe shows a marked blue-to-yellow emission color change in response to RSH, a significant two-photon cross section, good mitochondrial thiol selectivity, low cytotoxicity, and insensitivity to pH over the biologically relevant pH range, allowing the direct visualization of RSH levels in live cells as well as in living tissues at 90鈥?90 渭m depth without interference from other biologically relevant species through the use of two-photon microscopy.